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Synaptobrevin 2 - 104 211

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产品名称: Synaptobrevin 2 - 104 211
产品型号: 104 211
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Synaptobrevin 2 - 104 211


Synaptobrevin 2 - 104 211  的详细介绍
Cat. No. 104 211 50 µg purified IgG, lyophilized. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 10000 up to 1 : 100000 (AP staining) gallery  
IP: yes (see remarks)
ICC: 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P/FFPE: 1 : 500 gallery  
EM: yes
ELISA: yes (see remarks)
Clone 69.1
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 2 to 17 from rat Synaptobrevin2 (UniProt Id: P63045)
Epitop Epitop: AA 2 to 17 from rat Synaptobrevin2 (UniProt Id: P63045)
Reactivity Reacts with: human (P63027), rat (P63045), mouse (P63044), hamster.
No signal: chicken, zebrafish.
Other species not tested yet.
Specificity Specific for VAMP 2. No cross-reactivity to VAMP 1 and VAMP 3. (K.O. verified)
matching control protein/peptide 104-2P
Remarks

IP: This antibody quantitatively precipitates synaptobrevin 2 from detergent extracts regardless of whether the protein is associated.

ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 104 102 or 104 203 as detector antibodies (protocol for sandwich-ELISA).
Although the epitope for this antibody is still present in the Botulinumtoxin B cleavage product (aa 1 - 76), it is not recognized for unknown reasons.
Datasheet 104_211.pdf
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