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Glycine receptor - 146 308

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产品名称: Glycine receptor - 146 308
产品型号: 146 308
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Glycine receptor - 146 308


Glycine receptor - 146 308  的详细介绍
Cat. No. 146 308 50 µg purified IgG, lyophilized. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 (see remarks) gallery  
IHC-P/FFPE: not tested yet
Clone GpmAb4a
Subtype IgG2 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 457 from rat Glycine receptor α1 (UniProt Id: P07727)
Epitop Epitop: AA 96 to 105 from rat Glycine receptor α1 (UniProt Id: P07727)
Reactivity Reacts with: human (P23415, P23416, P48167 ), rat (P07727, P22771, P20781 ), mouse (Q64018, Q7TNC8, P48168 ), pig, zebrafish.
Other species not tested yet.
Specificity Specific for all glycine receptor subunits.
Remarks This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb4a. The constant regions of the heavy and light chains have been replaced with Guinea pig specific sequences. The antibody can therefore be used with standard anti-Guinea pig secondary reagents. It also carries a Strep-tag at the C-terminus of the heavy chain.The antibody has been expressed in mammalian cells.

IHC: Tissue sections require additional methanol/acetic acid treatment prior to antibody incubation. For details see Dumoulin A, Triller A & Dieudonné S (2001).
recommended protocol

Datasheet 146_308.pdf
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