WB: The antibody is less sensitive in westernblotting compared to the rabbit antibody. This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
ICC: This antibody is also suitable for the surface staining of living cells. After washingcells with bound antibodies, they can be fixed and visualized with secondaryreagents.
IHC: For best results use the protocol of Schneider Gasser et al., 2006.
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