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GABA-A receptor α1 - 224 204

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产品名称: GABA-A receptor α1 - 224 204
产品型号: 224 204
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GABA-A receptor α1 - 224 204


GABA-A receptor α1 - 224 204  的详细介绍
Cat. No. 224 204 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 2000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 200 up to 1 : 1000 (see remarks) gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P/FFPE: not tested yet
Immunogen Synthetic peptide corresponding to AA 28 to 43 from rat GABA-A receptor α1 (UniProt Id: P62813)
Reactivity Reacts with: rat (P62813), mouse (P62812).
Other species not tested yet.
Specificity Specific for GABA-A receptor α1.
matching control protein/peptide 224-2P
Remarks

WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

ICC: This antibody is PFA fixation sensitive, use only mild fixation (2% PFA). Best results are obtained by application on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondaryreagents.

IHC: This antibody requires antigen retrieval with pepsin according to:
Lorincz A & Nusser Z (2008).
recommended protocol
Datasheet 224_204.pdf
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