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GABA-A receptor α1 - 224 203

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产品名称: GABA-A receptor α1 - 224 203
产品型号: 224 203
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GABA-A receptor α1 - 224 203


GABA-A receptor α1 - 224 203  的详细介绍
Cat. No. 224 203 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 (see remarks) gallery  
IHC: 1 : 1000 up to 1 : 5000 (see remarks) gallery  
IHC-P/FFPE: not tested yet
Immunogen Synthetic peptide corresponding to AA 28 to 43 from rat GABA-A receptor α1 (UniProt Id: P62813)
Reactivity Reacts with: rat (P62813), mouse (P62812).
Other species not tested yet.
Specificity Specific for GABA-A receptor α1. (K.O. verified)
matching control protein/peptide 224-2P
Remarks

WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

ICC: This antibody is PFA fixation sensitive, use only mild fixation (2% PFA).Best results are obtained by application on living cells. After washingcells with bound antibodies, they can be fixed and visualized with secondaryreagents.

IHC: For best results use the protocol of Schneider Gasser et al., 2006.
Datasheet 224_203.pdf
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