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Goat-anti-mouse Fab fragments - 701-msCpH

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产品名称: Goat-anti-mouse Fab fragments - 701-msCpH
产品型号: 701-msCpH
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Goat-anti-mouse Fab fragments - 701-msCpH


Goat-anti-mouse Fab fragments - 701-msCpH  的详细介绍
Cat. No. 701-msCpH 50 µg purified Fab1 fragment, lyophilized, fluorescence-labeled with CypHer5E. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications ICC: yes (see remarks) gallery  
Label CypHer5E
Remarks

The pH sensitive dye only regaines its fluorescence after the acidification of the targeted cellular compartment.

ICC: Pre-incubate the primary antibody with a 2.5-fold excess of Fab-CpH conjugate (e.g., 2 µg of primary antibody with 5 µg of Fab fragments) in a total volume of 10 µl for 30 min at RT in the dark.
Adjust the antibody to the desired dilution and immediately use it for your internalization assay.
Datasheet 701-mscph.pdf
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