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Ca2+ channel N-type, α-1B subunit - 152 313

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产品名称: Ca2+ channel N-type, α-1B subunit - 152 313
产品型号: 152 313
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Ca2+ channel N-type, α-1B subunit - 152 313


Ca2+ channel N-type, α-1B subunit - 152 313  的详细介绍
Cat. No. 152 313 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (see remarks)
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 (see remarks) gallery  
IHC-P/FFPE: not tested yet
EM: not tested yet gallery  
Immunogen Recombinant protein corresponding to AA 2056 to 2336 from rat Ca2+ channel N-type α-1B (UniProt Id: Q02294)
Reactivity Reacts with: rat (Q02294), mouse (O55017).
Other species not tested yet.
Specificity Specific for Ca2+ channel α-1B.
matching control protein/peptide 152-3P
Remarks

WB: Antibody 1 (cat. no. 152 303) is recommended for this application.
Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen.
This protein tends to aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

IHC: This antibody requires mild fixation. recommended protocol
Datasheet 152_313.pdf
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