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Ca2+ channel N-type, α-1B subunit - 152 311

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产品名称: Ca2+ channel N-type, α-1B subunit - 152 311
产品型号: 152 311
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Ca2+ channel N-type, α-1B subunit - 152 311


Ca2+ channel N-type, α-1B subunit - 152 311  的详细介绍
Cat. No. 152 311 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
IHC-P/FFPE: not tested yet
Clone 163E3
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to AA 2056 to 2336 from rat Ca2+ channel N-type α-1B (UniProt Id: Q02294)
Epitop Epitop: AA 2074 to 2354 from rat Ca2+ channel N-type α-1B (UniProt Id: Q02294)
Reactivity Reacts with: rat, mouse.
Other species not tested yet.
Specificity Specific for Ca2+ channel α-1B.
matching control protein/peptide 152-3P
Remarks

WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen.
This protein tends to aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Datasheet 152_311.pdf
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