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Ca2+ channel P/Q-type, α-1A subunit - 152 205

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产品名称: Ca2+ channel P/Q-type, α-1A subunit - 152 205
产品型号: 152 205
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Ca2+ channel P/Q-type, α-1A subunit - 152 205


Ca2+ channel P/Q-type, α-1A subunit - 152 205  的详细介绍
Cat. No. 152 205 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Guinea pig serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 (see remarks) gallery  
IHC-P/FFPE: not tested yet
EM: yes gallery  
Immunogen Recombinant protein corresponding to AA 1921 to 2212 from rat Ca2+ channel P/Q-type α-1A (UniProt Id: P54282)
Reactivity Reacts with: rat (P54282), mouse (P97445).
Other species not tested yet.
Specificity Specific for Ca2+ channel α-1A. (K.O. verified)
Remarks

WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen.
This protein tends to aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

IHC: This antibody requires mild fixation. recommended protocol
Datasheet 152_205.pdf
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