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Ca2+ channel P/Q-type, α-1A subunit - 152 211

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产品名称: Ca2+ channel P/Q-type, α-1A subunit - 152 211
产品型号: 152 211
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Ca2+ channel P/Q-type, α-1A subunit - 152 211


Ca2+ channel P/Q-type, α-1A subunit - 152 211  的详细介绍
Cat. No. 152 211 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 100 up to 1 : 500 (see remarks) gallery  
IHC: not tested yet
IHC-P/FFPE: not tested yet
Clone 23F11
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to AA 1921 to 2212 from rat Ca2+ channel P/Q-type α-1A (UniProt Id: P54282)
Epitop Epitop: AA 1921 to 2212 from rat Ca2+ channel P/Q-type α-1A (UniProt Id: P54282)
Reactivity Reacts with: rat, mouse.
Other species not tested yet.
Specificity Specific for Ca2+ channel α-1A.
Remarks

WB: Less sensitive than the polyclonal antibodies. Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen.
This protein tends to aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

ICC: This antibody requires mild fixation (1-2% PFA for 15min).

Datasheet 152_211.pdf
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