WB: Less sensitive than the polyclonal antibodies. Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen.This protein tends to aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
ICC: This antibody requires mild fixation (1-2% PFA for 15min).
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