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VGLUT 1 - 135 311

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产品名称: VGLUT 1 - 135 311
产品型号: 135 311
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VGLUT 1 - 135 311


VGLUT 1 - 135 311  的详细介绍
Cat. No. 135 311 100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 500 up to 1 : 2000 (AP staining) (see remarks) gallery  
IP: yes (see remarks)
ICC: 1 : 100 gallery  
IHC: 1 : 100 gallery  
IHC-P/FFPE: 1 : 100 up to 1 : 500 gallery  
ELISA: yes (see remarks)
Clone 317D5
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to AA 456 to 560 from rat VGLUT1 (UniProt Id: Q62634)
Epitop Epitop: AA 542 to 560 from rat VGLUT1 (UniProt Id: Q62634)
Reactivity Reacts with: rat (Q62634), mouse (Q3TXX4).
Other species not tested yet.
Specificity Specific for VGLUT 1. (K.O. verified)
matching control protein/peptide 135-3P
Remarks

WB: This antibody yields stronger signals in Western blot experiments than cat. no. 135 511 but is less sensitive than cat. no. 135 011 and the polyclonal VGluT 1 antibodies.

IP: Coupling to protein A is recommended for IP, since covalent coupling to activated sepharose leads to considerable loss of activity.

ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 135 303 as detector antibody (protocol for sandwich-ELISA).
VGLUT 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Datasheet 135_311.pdf
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