Animal-Free Recombinant Human TWEAK

TWEAK belongs to the TNF family of ligands, and signals through TWEAKR, also known as TNFRSF12A. TWEAK is expressed in a variety of tissues, including the ***** heart, pancreas, skeletal muscle, small intestine, spleen and peripheral blood lymphocytes. TWEAK has the ability to induce NF-κB activation and chemokine secretion, and to exert an apoptotic activity in certain cells, such as HT-29 human adenocarcinoma cells when cultured in the presence of IFN-γ. TWEAK also promotes proliferation and migration of endothelial cells. The human TWEAK gene encodes for a 249 amino acid type II transmembrane protein, which contains a 21 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 207 amino acid extracellular domain. Recombinant Human TWEAK is a soluble 17.0 kDa polypeptide (154 amino acid residues) comprising the TNF-homologous region of TWEAK, and is generated by proteolytic processing of the full length membrane-anchored TWEAK protein.

Manufactured using all Animal-Free reagents.

Source: E.coli

Synonyms: TNF-related weak inducer of apoptosis, TNFSF12, DR3LG, Apo3 Ligand

AA Sequence: MKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Assay #1:  The ED₅₀ as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 10 ng/ml. 

Assay #2:  TWEAK weakly induces the death of HT29 cells when cultured in the presence of IFN-γ.  The ED₅₀ for this effect is between 30-45 ng/ml.

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