40nm NHS-Activated Silver Nanoparticle Conjugation Kit (10 reactions)

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产品名称: 40nm NHS-Activated Silver Nanoparticle Conjugation Kit (10 reactions)

产品型号: SN5K-40-2

产品展商: Cytodiagnostics

产品文档: 无相关文档

简单介绍

One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 40nm silver nanoparticles. Contains enough reagents for conjugation of 10 x 20ug of protein.

40nm NHS-Activated Silver Nanoparticle Conjugation Kit (10 reactions) 的详细介绍

Product Details

Cytodiagnostics 40nm NHS-activated Silver Nanoparticle Conjugation Kit have been optimized for high efficiency one-step conjugation of proteins and other primary amine-containing ligands to silver nanoparticles.

The kit contains ready-to-use pre-made mixtures. No activation or manipulation of the silver nanoparticles is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated NHS ester silver nanoparticles supplied with the kit.

Kit Components

40nm NHS-Activated Silver Nanoparticles (lyophilized)
Protein Resuspension Buffer
Reaction Buffer
Quencher Solution

Features & Benefits

Results in covalently bound ligand and more stable conjugate.
Fast and convenient one-step conjugation reaction with no pre-activation requirements.
Spacer between the silver nanoparticle surface and conjugated ligand minimizes effects on the tertiary protein structure, which can lead to poor performing conjugates, a common problem seen in conjugates prepared by passive adsorption.
Precisely engineering surface coating that reduces non-specific protein binding in biological assays.

Gold Nanoparticle Specifications

Surface functionality: NHS-ester (~ 1 NHS group/nm2) (10kDa PEG-spacer between silver surface and NHS-group)

Core diameter: 40nm

Concentration: OD=50 (when dissolved to a final volume of 100ul)

Particles per ml: ~1.1E+12 (when dissolved to a final volume of 100ul)

Absorbance (lambda max): 405-425nm

40nm-Silver-Nanoparticles-UV-Vis

Diameter	Peak SPR Wavelength (nm)	NPS/ml*	Wt. Conc (mg/ml)*	Size Dispersity (+/-nm)	Particle Volume (nm³)	Surface Area (nm²)	Surface/ Volume Ratio	Particle Mass (g)	Molar Mass (g/mol)	Molar Concentration*
40	405-425	~5.7E+10	2.00E-02	<15%	3.35E+04	5.03E+03	0.15	3.52E-16	2.12E+08	9.47E-11

Product Sheet

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