Gold Nanoparticle Conjugation Optimization Kit, (Dot-blot)

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产品名称: Gold Nanoparticle Conjugation Optimization Kit, (Dot-blot)

产品型号: GO-MEM-200

产品展商: Cytodiagnostics

产品文档: 无相关文档

简单介绍

Kit to optimize protein adsorption to Cytodiagnostics 20nm and 30nm gold nanoparticles for membrane blotting applications. Complete system with buffers and 100ml each of 20nm and 30 nm gold nanopartic

Gold Nanoparticle Conjugation Optimization Kit, (Dot-blot) 的详细介绍

Product Details

Supplied in Kit:

20nm and 30nm standard gold nanoparticles (100ml each, conc. OD1)
12 conjugation optimization buffers (1.5ml each)
10% NaCl (100ml)
Protein resuspension buffer
Detailed conjugation optimization protocol

Kit contains enough material to optimize 10 protein conjugates, or one optimization reaction and scale-up production of 20ml of conjugate at a final optical density of OD3.

Note: Only use with Cytodiagnostics standard or reactant free gold nanoparticles.

Gold nanoparticle conjugates have been used for a wide range of biological applications including the use as probes in light and electron microscopy (TEM) and for membrane blotting (immunogold dot-blot).

Stable gold nanoparticle conjugates can readily be prepared by passive adsorption of a protein to the gold nanoparticle surface, which is mediated by electrostatic and hydrophobic interactions between the protein and the surface layer of the colloidal gold.

Our gold nanoparticle protein conjugation optimization kits are supplied as complete and easy to use systems for increased success rates in the development of good quality gold nanoparticle conjugates.

Diameter	Peak SPR Wavelength (nm)	NPS/ml*	Wt. Conc (mg/ml)*	Molar Ext (M^-1cm^-1)	Size Dispersity (+/-nm)	Particle Volume (nm³)	Surface Area (nm²)	Surface/ Volume Ratio	Particle Mass (g)	Molar Mass (g/mol)	Molar Concentration*
20	524	6.54E+11	5.31E-02	9.21E+08	<12%	4.19E+03	1.26E+03	0.3	8.12E-17	4.89E+07	1.09E-09
*All concentrations as indicated at an optical density of 1 (OD=1)

Diameter	Peak SPR Wavelength (nm)	NPS/ml*	Wt. Conc (mg/ml)*	Molar Ext (M^-1cm^-1)	Size Dispersity (+/-nm)	Particle Volume (nm³)	Surface Area (nm²)	Surface/ Volume Ratio	Particle Mass (g)	Molar Mass (g/mol)	Molar Concentration*
30	526	1.79E+11	4.91E-02	3.36E+09	<12%	1.41E+04	2.83E+03	0.2	2.74E-16	1.65E+08	2.98E-10
*All concentrations as indicated at an optical density of 1 (OD=1)

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