RT-PCR Primers

Human COX6B Primer Pair for RT-PCR (reverse transcription followed by polymerase chain reaction) analysis of mRNA expression.

281bp

NM_001863

RT-PCR

The Primer Pair is stable for up to one year at
-20 °C in a non-frost free freezer。
Avoid repeated freeze-thaw cycles.

Primer Pairs for RT-PCR provide convenient
primers for analyzing the expression of specific mRNAs or got specific PCR fragment by RT-PCR. Each Primer Pair includes a 5´ and 3´ oligonucleotide primer provided in individual vials at 50μM ready for addition to the amplification reaction. The Primer Pairs have been confirmed in two-step conventional RT-PCR using human esophageal carcinoma EC109 cells total RNA.
PCR products from the use of the Primer Pairs have been sequence-confirmed for appropriate.

• Thaw all reagents completely on ice before use. • Use either random primers or oligo (dT) for reverse transcription. • All PCR reactions should be assembled on ice. • The recommended annealing