IP antibody use: 5ug V5 Mouse IgG1 per ml Lysate, WB 1: 5000 1) untransfected 293 cell lysate 2) transfected 293 cell lysate with V5-tag fusion protein 3) IP (untransfted 293+anti-V5 mAb+Protein G agarose) 4) IP (transfected 293+ normal Mouse IgG+Prote
IF analysis of 293 cells transfected with a V5-tag protein,1:2000 dilution (blue DAPI, red anti-V5)
1ug V5 fusion protein+ Primary antibody dilution at 1) 1:5000 2) 1:10000
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