IP antibody use: 5ug His Mouse IgG1 per ml Lysate, WB 1: 3000 1) untransfected 293 cell lysate 2) transfected 293 cell lysate with His-tag fusion protein 3) IP (untransfected 293+anti-His mAb+ Protein G agarose) 4) IP (transfected 293+ normal Mouse IgG

IF analysis of 293 cells transfected with a His-tag protein,1:1000 dilution (blue DAPI, red anti-His)

2ug His fusion protein+ Primary antibody dilution at 1) 1:5000 2) 1:10000