IP antibody use: 5ug HA Mouse IgG1 per ml Lysate, WB 1: 5000 1) untransfected 293 cell lysate 2) transfected 293 cell lysate with HA-tag fusion protein 3) IP (untransfted 293+anti-HA mAb+Protein G agarose) 4) IP (transfected 293+ normal Mouse IgG+Prote
IF analysis of 293 cells transfected with a HA-tag protein,1:2000 dilution (blue DAPI, red anti-HA)
0.5ug HA fusion protein+ Primary antibody dilution at 1:10000
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