IP antibody use: 5ug Flag Mouse IgG1 per ml Lysate, WB 1:5000 1) untransfected 293 cell lysate 2) transfected 293 cell lysate with Flag-tag fusion protein 3) IP (transfected 293+ normal Mouse IgG+Protein G agarose) 4) IP (transfected 293+anti- Flag mA
IF analysis of 293 cells transfected with a Flag-tag protein,1:2000 dilution (blue DAPI, red anti-Flag)
1ug Flag fusion protein+ Primary antibody dilution at 1:10000
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