Tag/Control Antibodies

DYKDDDDK(FLAG) synthetic peptide conjugate to KLH

mouse

ELISA,WB,IF,IP;Recommended dilution:WB:1:500-1:5000,IF:1:50-1:200,IP:1:200-1:2000

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

monoclonal

IgG2a

>95%,Protein G purified

Non-conjugated

Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

liquid

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

3X Flag, DDK tag, DYKDDDDK, Flag, Flag Tag