WB: Mouse Anti-GFP monoclonal antibody at 0.1ug/ml Line 1:HEK-293 cell lysate Line 2:GFP transfected HEK-293 cell lysate Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution Predicted band size : 30kd Observed band size : 30kd
IP:Result of anti-GFP-tag Monoclonal antibody Line 1:Control IgG Line 2:Precipitateing GFP-tagged fusion protein Line 3:GFP Transfected HEK-293 cells lysate Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution Predicted band size : 30kd Observed band size : 30kd
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