Western Blot
Positive WB detected in:Hela whole cell lysate,HEK293 whole cell lysate
All lanes : PODXL antibody at 2.5 ug/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa
Western Blot
Positive WB detected in:A549 whole cell lysate
All lanes : PODXL antibody at 2.5 ug/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa
Western Blot
Positive WB detected in:Mouse brain tissue
All lanes : PODXL antibody at 0.43 ug/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa
Western Blot
Positive WB detected in:Rat brain tissue,Rat heart tissue,Rat kidney tissue
All lanes : PODXL antibody at 1.28 ug/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa
Immunohistochemistry of paraffin-embedded Human kidney using CSB-MA134471A0m at dilution of 1:200
Immunohistochemistry of paraffin-embedded Human endometrial cancer using CSB-MA134471A0m at dilution of 1:200
Immunofluorescent analysis of Hela cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
Immunofluorescent analysis of PC-3 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
Immunofluorescent analysis of A549 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
Immunofluorescent analysis of Ntera2 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
Overlay histogram showing Hela cells stained with CSB-MA134471A0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10ug/1x106cells) for 1 h at 4℃. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4℃. Isotype control antibody (green line) was mouse IGg2b (10ug/1x106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Immunoprecipitating PODXL in HEK293 whole cell lysate
Lane 1: Rabbit monoclonal IgG(1 ug)instead of CSB-MA134471A0m in HEK293 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-MA134471A0m(8 ug)+ HEK293 whole cell lysate(500 ug)
Lane 3: HEK293 whole cell lysate (10 ug)
