Anti-DDDDK Tag Antibody

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产品名称: Anti-DDDDK Tag Antibody

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简单介绍

Anti-DDDDK Tag Antibody

Anti-DDDDK Tag Antibody 的详细介绍

Overview

Name:	Anti-DDDDK Tag Antibody See all DDDDK Tag primary antibodies
Description:	Mouse monoclonal DDDDK Tag antibody.
Applications:	WB, IHC, IF, IP
Dilutions:	WB: 1:2000 - 1:5000, IHC: 1:50 - 1:500, IF: 1:50 - 1:500, IP: 1:50 - 1:100.
Immunogen:	A synthetic Peptide of human DDDDK-Tag.
Host:	Mouse
Clonality:	Monoclonal
Isotype:	IgG
Conjugate:	Unconjugated
Purification:	Affinity purification.
Product Form:	Liquid
Formulation:	Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage:	Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Target

Background:

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a mouse monoclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins.

Synonyms:

DDDDK epitope tag Antibody

DDDDK tag Antibody

DDDK Antibody

ddk Antibody

DYKDDDDK Antibody

DYKDDDDK epitope tag Antibody

DYKDDDDK tag Antibody

ECS epitope tag Antibody

ECS tag Antibody

Enterokinase Cleavage Site epitope tag Antibody

Enterokinase Cleavage Site tag Antibody

FLAG Antibody

FLAG tag Antibody

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