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Anti-CRISPR-Cas9 Antibody

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产品名称: Anti-CRISPR-Cas9 Antibody
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Anti-CRISPR-Cas9 Antibody


Anti-CRISPR-Cas9 Antibody  的详细介绍
Name: Anti-CRISPR-Cas9 Antibody
See all CRISPR-Cas9 primary antibodies
Description: Mouse monoclonal antibody to CRISPR-Cas9
Specificity: This antibody detects recombinant Cas9 protein.
Applications: WB, ICC
Immunogen: This antibody is produced by immunizing mice with a recombinant protein corresponding to a sequence of CRISPR/Cas9.
Host: Mouse
Clonality: Monoclonal
Conjugate: Unconjugated
Molecular Weight: ~ 158 kDa
Purity: Protein A affinity purified
Product Form: Mouse IgM. 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Function: CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids) (PubMed:21455174). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA; Cas9 only stabilizes the pre-crRNA:tracrRNA interaction and has no catalytic function in RNA processing (PubMed:24270795). Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed 3'-5' exonucleolytically. DNA-binding requires protein and both gRNAs, as does nuclease activity. Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. DNA strand separation and heteroduplex formation starts at PAM sites; PAM recognition is required for catalytic activity (PubMed:24476820). Confers immunity against a plasmid with homology to the appropriate CRISPR spacer sequences (CRISPR interference) (PubMed:21455174).
Sequence Similarities: Belongs to the CRISPR-associated protein Cas9 family. Subtype II-A subfamily.
Biotechnological Use: Coexpression of Cas9 with an artifical single guide RNA (sgRNA) which fuses the crRNA with the tracrRNA in human cells has shown it is possible to target and modify DNA sequences of interest (PubMed:23287722, PubMed:23360966, PubMed:23386978). Cas9 plus the 2 sgRNAs have also been expressed individually in human and mouse cells to achieve DNA targeting; cleavage efficiencies of the artificial sgRNA were lower that those for systems with the 2 sgRNAs expressed separately (PubMed:23287718). Microinjection of Cas9-encoding mRNA and a synthetic sgRNA into zebrafish embryos induces targeted mutations (PubMed:23360964). In all cases introduction of multiple sgRNAs leads to multiplexed editing of genomic loci; DNA has also been inserted into a mammalian locus of interest. In S.pneumoniae and E.coli it has been used to generate markerless mutations; mutiple changes can be made simultaneously (PubMed:23360965). Studies to make mutations that alter the PAM-specificity and thus recognition possibilities have been made, but are not annotated in this database (PubMed:26098369).
Synonyms:
  • Cas9 Antibody
  • CRISPR-associated endonuclease Cas9/Csn1 Antibody
  • CRISPR-Cas9 Antibody
  • CRISPR-Cas9/Csn1 Antibody
  • csn1 Antibody
  • SpyCas9 Antibody
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    Information: Target information shown above is from the UniProt Consortium.
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