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Anti-C-RAF (Phospho-Tyr341) Antibody

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产品名称: Anti-C-RAF (Phospho-Tyr341) Antibody
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Anti-C-RAF (Phospho-Tyr341) Antibody


Anti-C-RAF (Phospho-Tyr341) Antibody  的详细介绍
Name: Anti-C-RAF (Phospho-Tyr341) Antibody
See all C-RAF primary antibodies
Description: Rabbit polyclonal antibody to C-RAF (Phospho-Tyr341)
Specificity: The antibody detects endogenous levels of Raf1 only when phosphorylated at tyrosine 341.
Applications: WB, IHC
Dilutions: Western blotting: 1:500~1:1000Immunohistochemistry: 1:50~1:100
Reactivity: Human
Immunogen: Peptide sequence around phosphorylation site of tyrosine 341 (S-Y-Y(p)-W-E) derived from Human C-RAF .
Host: Rabbit
Clonality: Polyclonal
Conjugate: Unconjugated
Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration: 1.0mg / ml
Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage: Store at -20?C / 1 year
Function: Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
Tissue Specificity: In skeletal muscle, isoform 1 is more abundant than isoform 2.
Involvement in Disease: Noonan syndrome 5: A form of Noonan syndrome, a disease characterized by short stature, facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears, and a high incidence of congenital heart defects and hypertrophic cardiomyopathy. Other features can include a short neck with webbing or redundancy of skin, deafness, motor delay, variable intellectual deficits, multiple skeletal defects, cryptorchidism, and bleeding diathesis. Individuals with Noonan syndrome are at risk of juvenile myelomonocytic leukemia, a myeloproliferative disorder characterized by excessive production of myelomonocytic cells.

LEOPARD syndrome 2: A disorder characterized by lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and sensorineural deafness.

Cardiomyopathy, dilated 1NN: A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Sequence Similarities: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Post-Translational Modification: Phosphorylation at Thr-269, Ser-338, Tyr-341, Thr-491 and Ser-494 results in its activation. Phosphorylation at Ser-29, Ser-43, Ser-289, Ser-296, Ser-301 and Ser-642 by MAPK1/ERK2 results in its inactivation. Phosphorylation at Ser-259 induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by the complex containing protein phosphatase 1, SHOC2 and M-Ras/MRAS relieves inactivation, leading to stimulate RAF1 activity. Phosphorylation at Ser-338 by PAK1 and PAK5 and Ser-339 by PAK1 is required for its mitochondrial localization. Phosphorylation at Ser-621 in response to growth factor treatment stabilizes the protein, possibly by preventing proteasomal degradation. Phosphorylation at Ser-289, Ser-296, Ser-301, Ser-338 and Ser-621 are somehow linked to the methylation potential of cells. Treatment of cells with HGF in the presence of the methylation inhibitor 5'-methylthioadenosine (MTA) results in increased phosphorylation at Ser-338 and Ser-621 and decreased phosphorylation at Ser-296, Ser-301 and Ser-338. Dephosphorylation at Ser-338 by PPP5C results in a activity decrease.
Cellular Location: Cytoplasm. Cell membrane. Mitochondrion. Nucleus.

Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. Phosphorylation at Ser-259 impairs its membrane accumulation. Recruited to the cell membrane by the active Ras protein. Phosphorylation at Ser-338 and Ser-339 by PAK1 is required for its mitochondrial localization. Retinoic acid-induced Ser-621 phosphorylated form of RAF1 is predominantly localized at the nucleus.
Database Links:
  • Entrez Gene: 5894?Human
  • Omim: 164760?Human
  • SwissProt: P04049?Human
  • Unigene: 159130?Human
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    Synonyms:
  • c Raf Antibody
  • C-raf Antibody
  • C-Raf proto-oncogene, serine/threonine kinase Antibody
  • CMD1NN Antibody
  • cRaf Antibody
  • Craf 1 transforming gene Antibody
  • Craf1 transforming gene Antibody
  • EC 2.7.11.1 Antibody
  • kinase Raf1 Antibody
  • Murine sarcoma 3611 oncogene 1 Antibody
  • NS5 Antibody
  • Oncogene MIL Antibody
  • Oncogene RAF1 Antibody
  • OTTHUMP00000160218 Antibody
  • OTTHUMP00000207813 Antibody
  • OTTHUMP00000209389 Antibody
  • Protein kinase raf 1 Antibody
  • Proto-oncogene c-RAF Antibody
  • RAF Antibody
  • Raf 1 Antibody
  • Raf 1 proto oncogene serine/threonine kinase Antibody
  • Raf proto oncogene serine/threonine protein kinase Antibody
  • RAF proto-oncogene serine/threonine-protein kinase Antibody
  • RAF-1 Antibody
  • Raf1 Antibody
  • RAF1_HUMAN Antibody
  • Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 Antibody
  • TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV Antibody
  • v raf 1 murine leukemia viral oncogene homolog 1 Antibody
  • v-raf murine sarcoma viral oncogene homolog 1 Antibody
  • v-raf-1 murine leukemia viral oncogene-like protein 1 Antibody
  • vraf1 murine leukemia viral oncogene homolog 1 Antibody
    		•
    Information: Target information shown above is from the UniProt Consortium.
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