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Anti-Aurora A (phospho-Thr288) Antibody

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产品名称: Anti-Aurora A (phospho-Thr288) Antibody
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Anti-Aurora A (phospho-Thr288) Antibody


Anti-Aurora A (phospho-Thr288) Antibody  的详细介绍
Name: Anti-Aurora A (phospho-Thr288) Antibody
See all Aurora A primary antibodies
Description: Rabbit polyclonal antibody to Aurora A (phospho-Thr288)
Specificity: The antibody detects endogenous level of Aurora A only when phosphorylated at threonine 288.
Applications: WB, IHC
Dilutions: Immunohistochemistry: 1:50~1:100
Reactivity: Human, Mouse
Immunogen: Peptide sequence around phosphorylation site of threonine 288 (R-T-T(p)-L-M) derived from Human Aurora A.
Host: Rabbit
Clonality: Polyclonal
Conjugate: Unconjugated
Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration: 1.0mg / ml
Predicted MW: 48kd
Formulation: Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage: Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Function: Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis.
Tissue Specificity: Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
Sequence Similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Post-Translational Modification: Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Thr-288 can be phosphorylated by several kinases, including PAK and PKA. Protein phosphatase type 1 (PP1) binds AURKA and inhibits its activity by dephosphorylating Thr-288 during mitosis. Phosphorylation at Ser-342 decreases the kinase activity. PPP2CA controls degradation by dephosphorylating Ser-51 at the end of mitosis.
Cellular Location: Cytoplasm > Cytoskeleton > Microtubule organizing center > Centrosome. Cytoplasm > Cytoskeleton > Spindle pole. Cytoplasm > Cytoskeleton > Cilium basal body. Cytoplasm > Cytoskeleton > Microtubule organizing center > Centrosome > Centriole.

Detected at the neurite hillock in developing neurons (By similarity). Localizes at the centrosome in mitotic cells from early prophase until telophase, but also localizes to the spindle pole MTs from prophase to anaphase (PubMed:9606188, PubMed:17229885, PubMed:21225229). Colocalized with SIRT2 at centrosome (PubMed:22014574). Moves to the midbody during both telophase and cytokinesis (PubMed:17726514). Associates with both the pericentriolar material (PCM) and centrioles (PubMed:22014574).
Database Links:
  • Entrez Gene: 6790?Human
  • Entrez Gene: 20878?Mouse
  • Omim: 603072?Human
  • SwissProt: O14965?Human
  • SwissProt: P97477?Mouse
  • Unigene: 250822?Human
  • Unigene: 249363?Mouse
    		•
    Synonyms:
  • AIK Antibody
  • ARK-1 Antibody
  • ARK1 Antibody
  • AURA Antibody
  • Aurka Antibody
  • Aurora 2 Antibody
  • Aurora A Antibody
  • Aurora kinase A Antibody
  • Aurora-related kinase 1 Antibody
  • Aurora/IPL1 like kinase Antibody
  • Aurora/IPL1-related kinase 1 Antibody
  • AURORA2 Antibody
  • Breast tumor-amplified kinase Antibody
  • BTAK Antibody
  • hARK1 Antibody
  • IAK Antibody
  • IPL1 related kinase Antibody
  • MGC34538 Antibody
  • OTTHUMP00000031340 Antibody
  • OTTHUMP00000031341 Antibody
  • OTTHUMP00000031342 Antibody
  • OTTHUMP00000031343 Antibody
  • OTTHUMP00000031344 Antibody
  • OTTHUMP00000031345 Antibody
  • OTTHUMP00000166071 Antibody
  • OTTHUMP00000166072 Antibody
  • PPP1R47 Antibody
  • Protein phosphatase 1, regulatory subunit 47 Antibody
  • Serine/threonine kinase 15 Antibody
  • Serine/threonine kinase 6 Antibody
  • Serine/threonine-protein kinase 15 Antibody
  • Serine/threonine-protein kinase 6 Antibody
  • Serine/threonine-protein kinase aurora-A Antibody
  • STK15 Antibody
  • STK6 Antibody
  • STK6_HUMAN Antibody
  • STK7 Antibody
    		•
    Information: Target information shown above is from the UniProt Consortium.
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