Human C Reactive Protein ELISA Kit (High Sensitivity)

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产品名称: Human C Reactive Protein ELISA Kit (High Sensitivity)

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Human C Reactive Protein ELISA Kit (High Sensitivity) 的详细介绍

Name:	Human C Reactive Protein ELISA Kit (High Sensitivity) See all C Reactive Protein kits
Applications:	Sandwich ELISA
Assay Type:	Sandwich (quantitative)
Platform:	Microplate
Detection Type:	Colorimetric
Sample Type:	Serum and plasma.
Sensitivity:	< 30 ng/ml
Range:	30-5000 ng/ml
Reactivity:	Human
Principle of Assay:	In order to quantitate the amount of CRP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated antibody specific for CRP is added to each well to "sandwich" the CRP immobilized during the second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CRP and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of an acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. In order to measure the concentration of CRP in the samples, this kit standard (ready-to use) is assayed at the same time as the samples (diluted if necessary with Sample Diluent). This allows the operator to produce a standard curve of Optical Density (O.D.) versus CRP concentration (mg/L). The concentration of CRP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Function:	Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
Tissue Specificity:	Found in plasma.
Sequence Similarities:	Belongs to the pentraxin family.
Cellular Location:	Secreted.
Database Links:	Entrez Gene: 1401 Human Omim: 123260 Human SwissProt: P02741 Human Unigene: 709456 Human Unigene: 76452 Human
Synonyms:	Pentraxin 1, short C Reactive Protein C reactive protein pentraxin related C-reactive protein(1-205) CRP CRP_HUMAN MGC88244 Pentraxin 1 PTX 1 PTX1
Information:	Protein information shown above is from the UniProt Consortium.

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