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Anti-Histone H2A.X (phospho-S139) Antibody

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产品名称: Anti-Histone H2A.X (phospho-S139) Antibody
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Anti-Histone H2A.X (phospho-S139) Antibody


Anti-Histone H2A.X (phospho-S139) Antibody  的详细介绍
Name: Anti-Histone H2A.X (phospho-S139) Antibody
See all Histone H2A.X primary antibodies
Description: Rabbit polyclonal antibody to Histone H2A.X (phospho-S139)
Specificity: p-Histone H2A.X (S139) pAb detects endogenous levels of Histone H2A.X protein only when phosphorylated at Ser139.
Applications: WB, IHC
Reactivity: Human, Mouse, Rat
Immunogen: Synthetic phosphopeptide derived from human Histone H2A.X around the phosphorylation site of Serine 139.
Host: Rabbit
Clonality: Polyclonal
Conjugate: Unconjugated
Molecular Weight: ~ 15 kDa
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Product Form: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Function: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Sequence Similarities: Belongs to the histone H2A family.
Post-Translational Modification: Phosphorylated on Ser-140 (to form gamma-H2AX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Phosphorylation can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. Widespread phosphorylation may also serve to amplify the damage signal or aid repair of persistent lesions. Phosphorylation of Ser-140 (H2AX139ph) in response to ionizing radiation is mediated by both ATM and PRKDC while defects in DNA replication induce Ser-140 phosphorylation (H2AX139ph) subsequent to activation of ATR and PRKDC. Dephosphorylation of Ser-140 by PP2A is required for DNA DSB repair. In meiosis, Ser-140 phosphorylation (H2AX139ph) may occur at synaptonemal complexes during leptotene as an ATM-dependent response to the formation of programmed DSBs by SPO11. Ser-140 phosphorylation (H2AX139ph) may subsequently occurs at unsynapsed regions of both autosomes and the XY bivalent during zygotene, downstream of ATR and BRCA1 activation. Ser-140 phosphorylation (H2AX139ph) may also be required for transcriptional repression of unsynapsed chromatin and meiotic sex chromosome inactivation (MSCI), whereby the X and Y chromosomes condense in pachytene to form the heterochromatic XY-body. During immunoglobulin class switch recombination in lymphocytes, Ser-140 phosphorylation (H2AX139ph) may occur at sites of DNA-recombination subsequent to activation of the activation-induced cytidine deaminase AICDA. Phosphorylation at Tyr-143 (H2AXY142ph) by BAZ1B/WSTF determines the relative recruitment of either DNA repair or pro-apoptotic factors. Phosphorylation at Tyr-143 (H2AXY142ph) favors the recruitment of APBB1/FE65 and pro-apoptosis factors such as MAPK8/JNK1, triggering apoptosis. In contrast, dephosphorylation of Tyr-143 by EYA proteins (EYA1, EYA2, EYA3 or EYA4) favors the recruitment of MDC1-containing DNA repair complexes to the tail of phosphorylated Ser-140 (H2AX139ph).
Cellular Location: Nucleus. Chromosome.
Database Links:
  • Entrez Gene: 3014?Human
  • Entrez Gene: 8337?Human
  • Entrez Gene: 8338?Human
  • Entrez Gene: 85235?Human
  • Entrez Gene: 9555?Human
  • Entrez Gene: 15270?Mouse
  • Entrez Gene: 500987?Rat
  • Entrez Gene: 64646?Rat
  • Omim: 142720?Human
  • Omim: 142763?Human
  • Omim: 300445?Human
  • Omim: 601772?Human
  • SwissProt: P16104?Human
  • SwissProt: Q16777?Human
  • SwissProt: Q99878?Human
  • SwissProt: P22752?Mouse
  • SwissProt: P27661?Mouse
  • SwissProt: P02262?Rat
  • SwissProt: Q64598?Rat
  • Unigene: 477879?Human
  • Unigene: 245931?Mouse
  • Unigene: 140775?Rat
  • Unigene: 2850?Rat
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    Synonyms:
  • AW228881 Antibody
  • H2A histone family member X Antibody
  • H2A.FX Antibody
  • H2A.X Antibody
  • H2a/x Antibody
  • H2AFX Antibody
  • H2AX Antibody
  • H2AX histone Antibody
  • H2AX_HUMAN Antibody
  • Hist5.2ax Antibody
  • Histone 2A Antibody
  • Histone 2AX Antibody
  • Histone H2A.X Antibody
  • Histone H2AX Antibody
  • RGD1566119 Antibody
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    Information: Target information shown above is from the UniProt Consortium.
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