7AAD Solution

7-Amino-Actinomycin D (7-AAD) is ready-to-use nucleic acid dye solution (2 ml). 7-AAD can be used in place of propidium iodide (PI) for the exclusion of nonviable cells in flow cytometry analysis. This product can be used in combination with PE (phycoerythrin), and FITC (Fluorescein isothiocyanate) conjugated antibodies in 2-color analysis. The advantage of 7-AAD over PI is the minimal spectral overlap between these emissions. Fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).

Aplication Reference:

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Sakkas A. et al. Safety and Efficacy of Suicide Gene Therapy with Adenosine Deaminase 5-Fluorocytosine Silmutaneously in in Vitro Cultures of Melanoma and Retinal Cell Lines. J Cancer. 2014; 5(5): 368–381

Piscianz E. et al. Fate of Lymphocytes after Withdrawal of Tofacitinib Treatment. PLoS One. 2014; 9(1): e85463.

Suarez-Alvarez B et al. Epigenetic Mechanisms Regulate MHC and Antigen Processing Molecules in Human Embryonic and Induced Pluripotent Stem Cells. PLoS One. 2010; 5(4): e10192.

Vela L et al. Iminophosphorane-organogold(III) complexes induce cell death through mitochondrial ROS production. J Inorg Biochem. 2011 Oct; 105(10): 1306–1313.

Frik M et al. Cyclometalated Iminophosphorane Gold(III) and Platinum(II) Complexes. A Highly Permeable Cationic Platinum(II) Compound with Promising Anticancer Properties. J Med Chem. 2015 Aug 13; 58(15): 5825–5841

Serrano-Pertierra E. et al. NKG2D- and CD28-mediated costimulation regulate CD8+ T cell chemotaxis through different mechanisms: the role of Cdc42/N-WASp. Journal of Leukocyte Biology. vol. 95 no. 3 487-495

Ruiz-Garcia R . Et al. Decreased activation-induced cell death by EBV-transformed B-cells from a patient with autoimmune lymphoproliferative syndrome caused by a novel FASLG mutation. 2015 PEDIATRIC RESEARCH

• Other names: 7AAD, 7-AAD, 7-AMINO-ACTINOMYCIN D