PE Annexin V

Apoptosis is characterized by a variety of morphological features. One of the earliest indications of apoptosis is the translocation of the membrane phospholipid phosphatidylserine (PS) from the inner to the outer leaflet of the plasma membrane. Once exposed to the extracellular environment, s on PS become available for Annexin V, a 35-36 kDa, Ca 2+-dependent, phospholipid binding protein with a high affinity for PS. The translocation of PS precedes other apoptotic processes such as loss of plasma membrane integrity, DNA fragmentation, and chromatin condensation. As such, Annexin V can be conjugated to biotin or to a fluorochrome, and used for the easy, flow cytometric identification of cells in the early stages of apoptosis.

Aplication Reference:

Jarauta V. et al. Inhibition of autophagy with chloroquine potentiates carfilzomib-induced apoptosis in myeloma cells in vitro and in vivo. 2016 Cancer Letters.

Rodríguez‑Mora et al. Intracellular expression of Tat alters mitochondrial functions in T cells: a potential mechanism to understand mitochondrial damage during HIV‑1 replication.  Retrovirology (2015) 12:78 DOI 10.1186/s12977-015-0203-3

Montes M. et al. Functional Consequences for Apoptosis by Transcription Elongation Regulator 1 (TCERG1)-Mediated Bcl-x and Fas/CD95 Alternative Splicing. PLoS One. 2015; 10(10): e0139812.

Vela L et al. Direct Interaction of Bax and Bak Proteins with Bcl-2 Homology Domain 3 (BH3)-only Proteins in Living Cells Revealed by Fluorescence Complementation. J Biol Chem. 2013 Feb 15; 288(7): 4935–4946. (Mouse)

Mazzeo C. et al. Protein kinase D1/2 is involved in the maturation of multivesicular bodies and secretion of exosomes in T and B lymphocytes. Cell Death and Differentiation (2016) 23, 99–109; doi:10.1038/cdd.2015.72

Ramirez-Labrada A et al. Two death pathways induced by sorafenib in myeloma cells: Puma-mediated apoptosis and necroptosis. 2015 Clin Transl Oncol

• Other names: Annexin A5 
• GeneID: 308
• Molecular weight: 35,9 kDa