FITC Annexin V

Apoptosis is characterized by a variety of morphological features. One of the earliest indications of apoptosis is the translocation of the membrane phospholipid phosphatidylserine (PS) from the inner to the outer leaflet of the plasma membrane. Once exposed to the extracellular environment, s on PS become available for Annexin V, a 35-36 kDa, Ca 2+-dependent, phospholipid binding protein with a high affinity for PS. The translocation of PS precedes other apoptotic processes such as loss of plasma membrane integrity, DNA fragmentation, and chromatin condensation. As such, Annexin V can be conjugated to biotin or to a fluorochrome, and used for the easy, flow cytometric identification of cells in the early stages of apoptosis.

Aplication Reference:

Jarauta V. et al. Inhibition of autophagy with chloroquine potentiates carfilzomib-induced apoptosis in myeloma cells in vitro and in vivo. 2016 Cancer Letter.

Marcos-Ramiro et al. Microparticles in multiple sclerosis and clinically isolated syndrome: effect on endothelial barrier function. 2014. BioMed Central Ltd

Moro L. et al. Placental Microparticles and MicroRNAs in Pregnant Women with Plasmodium falciparum or HIV Infection. PLoS One. 2016; 11(1): e0146361.

Monsalve et. Al. Human VRK2 modulates apoptosis by interaction with Bcl-xL and regulation of BAX gene expression. Cell Death and Disease (2013) 4, e513; doi:10.1038/cddis.2013.40

Marcos-Ramiro B et al. Microparticles in multiple sclerosis and clinically isolated syndrome: effect on endothelial barrier function. BMC Neurosci. 2014; 15: 110.

Lourenço B et al. Relation of Cumulus Cell Status with Single Oocyte Maturity, Fertilization Capability and Patient Age. J Reprod Infertil. 2014 Jan-Mar; 15(1): 15–21

Fernández-Guizán A. et al. Pleiotropic Anti-Angiogenic and Anti-Oncogenic Activities of the Novel Mithralog Demycarosyl-3D-ß-D-Digitoxosyl-Mithramycin SK (EC-8042). PLoS One. 2015; 10(11): e0140786

Acebes-Huerta A. et al. Lenalidomide Induces Immunomodulation in Chronic Lymphocytic Leukemia and Enhances Antitumor Immune Responses Mediated by NK and CD4 T Cells. Biomed Res Int. 2014; 2014: 265840

Amigo-Jiménez I et al. Matrix Metalloproteinase-9 Is Involved in Chronic Lymphocytic Leukemia Cell Response to Fludarabine and Arsenic Trioxide. PLoS One. 2014; 9(6): e99993.

Montes M. et al. Functional Consequences for Apoptosis by Transcription Elongation Regulator 1 (TCERG1)-Mediated Bcl-x and Fas/CD95 Alternative Splicing. PLoS One. 2015; 10(10): e0139812.

Ranera B. et al. Effect of hypoxia on equine mesenchymal stem cells derived from bone marrow and adipose tissue. BMC Vet Res. 2012; 8: 142.

Pericacho M. et al. Diminished Thrombogenic Responses by Deletion of the Podocalyxin Gene in Mouse Megakaryocytes. PLoS One. 2011; 6(10): e26025.

Espino J. et al. Melatonin is able to delay endoplasmic reticulum stress-induced apoptosis in leukocytes from elderly humans. Age (Dordr). 2011 Dec; 33(4): 497–507.

Martinez-Feito A et al. Autoimmune lymphoproliferative syndrome due to somatic FAS mutation (ALPS-sFAS) combined with a germline caspase-10 (CASP10) variation. Immunobiology. Volume 221, Issue 1, January 2016, Pages 40–47

Correia M. et al. Different concentrations of kaempferol distinctly modulate murine embryonic stem cell function. Food and Chemical Toxicology Volume 87, January 2016, Pages 148–156

Vilariño-Feltrer G. et al. Schwann-cell cylinders grown inside hyaluronic-acid tubular scaffolds with gradient porosity. 1742-7061/  2015 Acta Materialia Inc. Published by Elsevier Ltd

Ramirez-Labrada A et al. Two death pathways induced by sorafenib in myeloma cells: Puma-mediated apoptosis and necroptosis. 2015 Clin Transl Oncol

Murtinho D et al. Synthesis, characterization and assessment of the cytotoxic activity of Cu(II), Fe(III) and Mn(III) complexes of camphoric acid-derived salen ligands. 2015 Applied Organometallic Chemistry

• Other names: Annexin A5 
• GeneID: 308
• Molecular weight: 35,9 kDa