Protein Enrichment through Albumin Removal
Fast Results - Remove Albumin from Human Serum Samples in 30 minutes or less
Albumin removal tested and successfully removed from human, mouse, rat, sheep, goat and rabbit serum.
The BioMag® ProMax Albumin Removal Kit enables the quick removal of human serum albumin from samples. Based on BioMag® superparamagnetic particle technology, BioMag® ProMax Albumin Removal particles supplied in the kit, along with optimized buffers, allow for the selective binding and release of the less abundant proteins in the serum. The protocol can be completed in 30 minutes or less, so researchers can move on to analyzing important biomarkers in the serum. The BioMag® ProMax protocol is scalable and can be used in conjunction with the ProMax Serum IgG Removal Kit (Cat. #24352-1).
The BioMag® ProMax Albumin Removal Kit is supplied with particles and buffers for carrying out 25 separation reactions.
Figure 1
SDS-PAGE analysis and Western Blot showing enrichment of lower abundance proteins and depletion of albumin.
Panel A
Shows a silver stained SDS-PAGE gel. Lane 1, Molecular Weight Markers; Lane 2, untreated normal human serum; Lane 3, serum treated with ProMax Albumin Removal Particles. Both Lanes 2 and 3 were loaded with the same amount of protein.
Panel B
Shows the depletion of albumin by Western Blot. Lane 1, normal serum; Lane 2, serum treated with the BioMag® ProMax Albumin Removal Kit; Lane 3, Molecular Weight Markers. Mouse anti-albumin was the primary antibody and the signal was visualized using an anti-mouse horseradish peroxidase conjugate and TMB as the chromagen. Both Lanes 1 and 2 were loaded with equal amounts of protein. Lane 2 shows that nearly all of the albumin has been depleted from the sample.
Based on patented BioMag® particle technology
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