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Human 5a-Androstane-3a, 17ß diol Glucuronide (3a-Diol G) ELISA Kit

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产品名称: Human 5a-Androstane-3a, 17ß diol Glucuronide (3a-Diol G) ELISA Kit
产品型号: EKD01001
产品展商: Biomatik
产品文档: 无相关文档

简单介绍

Human 5a-Androstane-3a, 17ß diol Glucuronide (3a-Diol G) ELISA Kit


Human 5a-Androstane-3a, 17ß diol Glucuronide (3a-Diol G) ELISA Kit  的详细介绍

Product Description

Species Reactivity Homo sapiens (Human)
Format 96 well plate
Detection Range 0-50 ng/ml
Sensitivity 0.1 ng/ml
Sample Type Human Serum
Assay Time 2 hours
Application ELISA
Assay Type Competitive
Detection Method Colorimetric
Shipping Condition Ambient shipping
Storage Instructions 4°C. Bring all reagents to room temperature before beginning test. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Precaution Of Use The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Expiry Date 1 year
Test Principle The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabeled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody `s on the microwell plate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of 3a Diol G in the sample. A set of standards is used to plot a standard curve from which the amount of 3a Diol G in patient samples and controls can be directly read.
Assay Precision The intra assay precision is between a CV% of 6.0- 7.8 pg/ml. The inter assay precision is between a CV% of6.5-10.8pg/ml
Restrictions For research use only. Not for diagnostic procedures.
Quality Systems The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

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