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Androstenedione (ASD) Instant ELISA Kit

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产品名称: Androstenedione (ASD) Instant ELISA Kit
产品型号: EKU10329
产品展商: 其它品牌
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简单介绍

Androstenedione (ASD) Instant ELISA Kit


Androstenedione (ASD) Instant ELISA Kit  的详细介绍

Product Description

Species Reactivity Multispecies (General)
Format 96 well plate
Assay Time 1 hours, 10min
Gene Name Androstenedione
Gene Aliases 4-Androstenedione; 4-Androstene-3,17-Dione
Application ELISA
Assay Type Sandwich
Detection Method Colorimetric
Shipping Condition Blue ice
Storage Instructions -20 °C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Precaution Of Use The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Expiry Date 8 months
Specificity This assay has high sensitivity and excellent specificity for detection of Androstenedione (ASD). No significant cross-reactivity or interference between Androstenedione (ASD) and analogues was observed.
Test Principle The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Androstenedione (ASD). Standards or samples and HRP-labeled detection antibody specific to Androstenedione (ASD) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Androstenedione (ASD), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Androstenedione (ASD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Precision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Androstenedione (ASD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high leve
Stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions For research use only. Not for diagnostic procedures.
Quality Systems The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research Area Endocrinology;Reproductive science;Hormone metabolism;
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