BioGrammatics

具有改性糖基化的巴斯德毕赤酵母菌株--GlycoSwitch®技术。

BioGrammatics与Research Corporation Technologies（RCT）合作，现在提供**个Pichia表达载体，菌株和服务，以使用GlycoSwitch®在Pichia中修饰糖基化。GlycoSwitch®技术来自于比利时根特大学和法兰德斯生物技术研究所（VIB）的Roland Contreras博士，Nico Callewaert博士和同事，以及BioGrammatics*近的贡献。糖基化可影响蛋白质折叠，稳定性和蛋白质 - 蛋白质相互作用; 因此，GlycoSwitch®被设计用于产生具有更多“人类”糖基化的蛋白质以产生更好的生物药物，以及改善其他类型的重组蛋白质。

核糖GlycoSwitch®菌株具有**聚糖加工酶（OCH1），其破坏以防止聚糖延伸，并表达外源甘露糖苷酶以将现有聚糖修剪成更均匀的Man5结构。这些SuperMan5菌株可用于运送到您的实验室，以及表达载体也可进一步修饰糖基化途径。使用GlycoSwitch®技术的合同研发服务现在也可以通过BioGrammatics提供的所有Pichia专业知识为您的具体应用量身定做糖蛋白。

部分产品信息如下：

Category: Expression Vectors

Initial Tab: Overview

Pichia Expression Vectors.

Recombinant protein expression in Pichia is most common from strains with expression vectors integrated into the Pichia genome. Genomic integration results in stable strains for fermentation at which Pichia excels. Expression strains can be engineered to secrete proteins into a defined medium, at multiple grams/liter - on demand. However, each gene and the related expression project goals are unique and may benefit from an alternative expression system. Factors to consider include: 1) intracellular versus secreted expression 2) what kind of promoter (inducible, constitutive, strong, weak) 3) the selection marker 4) the specific gene/ORF sequence, and 5) the location of the expression cassette in the Pichia genome.

BioGrammatics has created and tested numerous expression vectors to optimize the expression of hundreds of genes for specific applications including both intracellular and extracellular expression, the expression of membrane proteins, expression for high-level protein production, as well as the expression of proteins in metabolic pathways and proteins that support optimal processing of a recombinant protein product. Please contact BioGrammatics with inquiries on specific expression vector needs (info@biogrammatics.com).

Following are a core set of BioGrammatics expression vectors designed for tightly regulated, strongly inducible expression with the Pichia Alcohol Oxidase I promoter (pAOX1). Even toxic genes can be expressed with the AOX1 promoter because little, if any, protein is made in Pichia prior to methanol induction. This core set includes vectors with, and without, a secretion signal (s1) and three different selectable markers (markers for G418, Noursethricin/Nat, or zeocin resistance). BioGrammatics recommends our pJAG and pJAN vectors for most initial expression tests. These vectors are available for immediate delivery.

All of BioGrammatics pJ – vectors (Jeannies vectors) are 4-5 kb plasmids with the ampicillin gene for selection in E. coli and a “seamless” cloning scheme. The cloning scheme is based on type IIS restriction enzymes that cut outside of their recognition sequence; therefore, restriction enzyme s are removed in the final expression vector and an ORF can be added without unwanted codons.

Product	Description
pJAN–s1	extracellular expression vector, S. cerevisiae α mating factor leader, nourseothricin selection
pJAZ–s1	extracellular expression vector, S. cerevisiae α mating factor leader, zeocin selection
pJAG–s1	extracellular expression vector, S. cerevisiae α mating factor leader, G418 selection
pJAN	intracellular expression vector, nourseothricin selection
pJAZ	intracellular expression vector, zeocin selection
pJAG	intracellular expression vector, G418 selection

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