MRC-Holland bv是一家位于荷兰阿姆斯特丹的生物技术公司。该公司是荷兰微生物研究中心的后续工作，1985年由Jan Schouten博士创立。直到2000年，MRC-Holland的主要产品是限制性内切核酸酶，从广泛的细菌和蓝绿色藻类和DNA分子量标记物中提取。这些产品大量销售给全球分销公司。
1997年，MRC-Holland开始专注于开发新的DNA分析技术。迄今为止开发的*成功的技术是多重连接探针扩增，或MLPA ®。所述MLPA ®技术*初是在描述核酸研究30，E57（2002）通过的Schouten等人。从那时起，已经开发了数百种MLPA探针组用于各种基因和应用，其中一些非常罕见。
目前，MRC-Holland的所有活动都针对生产新的基于MLPA的产品和开发基于MLPA的新技术。MRC-Holland目前拥有约70名员工，从事开发，质量检测，生产，销售和客户服务。
部分产品信息如下:
SALSA MLPA probemixes for DNA and methylation
# - 025r SALSA MLPA probemix - 25 reactions
# - 050r SALSA MLPA probemix - 50 reactions
# - 100r SALSA MLPA probemix - 100 reactions
P200-100r
P300-100r P200 or P300 Human DNA reference probemixes to use in combination with your own home-made probes.
The P200 and P300 probemixes are available only per 100 reactions
SALSA MLPA reagent kits
EK1-FAM* SALSA MLPA EK1 reagent kit
- 100 reactions (6 vials) - FAM
EK1-Cy5 SALSA MLPA EK1 reagent kit
- 100 reactions (6 vials) - Cy5.0
EK5-FAM SALSA MLPA EK5 reagent kit
- 500 reactions (5x6 vials) - FAM
EK5-Cy5 SALSA MLPA EK5 reagent kit
- 500 reactions (5x6 vials) - Cy5.0
EK20-FAM SALSA MLPA EK20 reagent kit
- 2000 reactions (5x6 vials) - FAM
*Unlabelled primers are available on request.
SALSA Sample DNA
SD### SALSA Binning DNA - 6 reactions (30 µl)*
SD### SALSA Artificial Duplication DNA - 20 reactions (100 µl)
SD### SALSA Reference DNA - 20 reactions (100 µl)**
*One free vial of Binning DNA is provided with every order of the relevant probemix.
**When this is stated in the product description of the probemix, one free vial of Reference DNA is provided with every order of that probemix.
SALSA MLPA reagents
SMR04 SALSA Sample Stabilising Solution (S4), 200 µl
SMR11 SALSA Ligase-65, 115 µl
SMR12 Ligase Buffer A, 360 µl
SMR13 Ligase Buffer B, 360 µl
SMR14 SALSA PCR buffer, 480 µl
SMR15 SALSA PCR Primer FAM, 240 µl
SMR16 SALSA Polymerase, 65 µl
SMR18 SALSA PCR Primer Cy5.0, 240 µl
SMR32 SALSA MLPA buffer, 180 µl
SMR45 SALSA Sample Stabilising Solution (S4), 1000 µl
Seperate SALSA MLPA reagents can only be ordered when they can be combined together in one product box.
SALSA PCR reagents
PCR001-FAM SALSA PCR reagents for 100 reactions - FAM
PCR001-CY5 SALSA PCR reagents for 100 reactions - Cy5
PCR003-FAM SALSA PCR reagents for 300 reactions - FAM
PCR003-CY5 SALSA PCR reagents for 300 reactions - Cy5
SALSA polymerase (substitute for Taq polymerase)
SP001 SALSA polymerase 2,500 units
+ 4 x 1.1 ml SALSA PCR buffer
SP002 SALSA polymerase 5,000 units
+ 8 x 1.1 ml SALSA PCR buffer
SP008 SALSA polymerase 20,000 units
+ 32 x 1.1 ml SALSA PCR buffer
SP016 SALSA polymerase: 40,000 units
+ 64 x 1.1 ml SALSA PCR buffer
Ligase-65
Lig-5a 550 µl SALSA Ligase-65 with MLPA buffer, Buffer A and Buffer B
Lig-10 2x 550 µl SALSA Ligase-65
Lig-50 10x 550 µl SALSA Ligase-65
Control fragments
CF1 Q+92-control fragments 175 µl for 3,000 reactions
CF4 Q+D-control fragments 175 µl for 3,000 reactions
