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MITC Inactivated Rat Embryonic Fibroblast (REF) Feeder Cells

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产品名称: MITC Inactivated Rat Embryonic Fibroblast (REF) Feeder Cells
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产品展商: ABM
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MITC Inactivated Rat Embryonic Fibroblast (REF) Feeder Cells


MITC Inactivated Rat Embryonic Fibroblast (REF) Feeder Cells  的详细介绍
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Safety BioSafety Level II
Organism Rat
Source Organ Embryo
Growth Properties Adherent
Morphology Fibroblast-like
Applications For Research Use Only. The inactivated REFs have been tested to be significantly better than inactivated MEF in supporting human iPSC reprogramming and long-term undifferentiated proliferation of human iPS cells and human ground state naive pluripotent stem cells. There is no need to change medium at weekend if human iPS or ES cells are split on Friday and cultured on this REF feeder line in iPSC Culture Medium (TM020).
Propagation The base medium for this cell line is Prigrow IV medium available from ABM (TM004). To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 15%; 0.1 mM Non-Essential Amino Acids; and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO2: 5%; Temperature: 37.0°C.
Storage -180°C
Shipping On Dry Ice
Notes For Research Use Only, not for therapeutic or diagnostic purposes.
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