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Pat5 Embryonic Stem Cells

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产品名称: Pat5 Embryonic Stem Cells
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产品展商: ABM
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Pat5 Embryonic Stem Cells


Pat5 Embryonic Stem Cells  的详细介绍
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Safety BioSafety Level II
Organism 129X1/SvJ mouse
Source Organ Embryo
Description Pat5 Embryonic Stem Cells were derived from 129X1/SvJ mouse embryos and can be utilized to introduce genetic mutations with gene targeting vectors through homologous recombination.

Growth Properties: Adherent
Morphology: Polygonal
Concentration 106cells/vial
Propagation Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. Cell line must be maintained on γ-irradiated mouse embryonic fibroblasts (T2003). The base medium for this cell line is Prigrow III medium available in abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10% and 1% Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO₂: 5%; Temperature: 37.0°C.
Storage -180°C
Shipping On Dry Ice
Notes For Research Use Only, not for therapeutic or diagnostic purposes.
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