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Immortalized-Human-Endocervical-Epithelial-Cells--HPV-E6\/E7-(A2EN)

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产品名称: Immortalized-Human-Endocervical-Epithelial-Cells--HPV-E6\/E7-(A2EN)
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产品展商: ABM
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Immortalized-Human-Endocervical-Epithelial-Cells--HPV-E6\/E7-(A2EN)


Immortalized-Human-Endocervical-Epithelial-Cells--HPV-E6\/E7-(A2EN)  的详细介绍
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BioSafety Level II
Organism Homo sapiens
Source Organ Endocervix
Growth Properties Adherent
Morphology Cobblestone
Recommended Seeding Density Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers MUC5B
Applications For Research Use Only
Immortalization Method Serial passaging and transduction with retroviruses carrying Human papillomavirus E6/E7 (type 16) protein (pLXSN)
Description The endocervical epithelium lines the endocervix in the female reproductive tract, separating sterile and microbe-rich regions from one another. Since the endocervix is a target for sexually transmitted organisms such as Chlamydia trachomatis and Neisseria gonorrhoeae, the endoecervical epithelial cells play an important role in both host defense and reproduction. The Immortalized Endocervical Epithelial Cells – HPV E6/E7 is unique in that it can be polarized to exhibit distinct apical and basolateral membrane domains and that it retained its ability to respond to hormone and microbial stimulus. A2EN cells serve as an excellent model to further characterization of pathogen-host interactions on sexually transmitted infections.
Procedure Overview
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Propagation Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow X.1 medium available in ABM, Cat. No. TM010. To make the complete growth medium, add the entire content of the growth supplements to the basal media (no additional fetal bovine serum is required) and Penicillin/Streptomycin (G255). Atmosphere: air, 95%; Carbon dioxide (CO2), 5%.
Preservation 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control (1) Electron microscopy was performed to assess cell polarization, while fluorescent microscopy was performed to assess tight junction formation between cells. (2) Transepithelial electrical resistance was measured to measure cell monolayer functional integrity. (3) RT-PCR, Western blotting, and immunostaining were performed to compare immortalized cell characteristics to normal counterparts. (4) Expression levels of antimicrobial peptides, cytokines, and chemokines were measured to further assess immortalized cell characteristics. (5) qPCR was performed to analyze toll-like receptor protein expression between basolateral and apical components of cells, while qRT-PCR was performed to determine hormone receptor functionality.
Disclaimer 1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.
2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).
3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.
4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.
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