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SLC35A1 CRISPR Knock Out CHO-S Cell Line

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产品名称: SLC35A1 CRISPR Knock Out CHO-S Cell Line
产品型号: T6040
产品展商: ABM
产品文档: 无相关文档

简单介绍

SLC35A1 CRISPR Knock Out CHO-S Cell Line


SLC35A1 CRISPR Knock Out CHO-S Cell Line  的详细介绍
Print Version
BioSafety Level II
Organism Chinese Hamster
SourceOrgan Ovary
Growth Properties Suspension
Morphology Round
Description SLC35A1 encodes a CMP-sialic acid transporter located within the membrane of the Golgi apparatus. CHO-S cells isolated from Chinese Hamster ovary were transfected with CRISPR/Cas9 plasmids which target SLC35A1 to generate the SLC35A1 CRISPR Knock Out CHO-S Cell Line (Hamster). This sialic acid-deficient cell line is useful for research focusing on SLC35A1 as it functions in transporting nucleotide sugars into the Golgi where glycosyltransferases function. The SLC35A1 CRISPR Knock Out CHO-S Cell Line (Hamster) is also useful in characterization of anti-glycopeptide monoclonal antibodies.
Procedure Overview
Recommended Seeding Density Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications For Research Use Only
Knockdown Method Transfecting CRISPR/Cas9 plasmids which target SLC35A1
Propagation The base medium for this cell line is CHO-S-SFM II medium (Thermo Fisher Scientific Inc). Recommend to add 1% Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO₂: 5%; Temperature: 37.0°C.
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Disclaimer 1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final.
2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).
3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.
4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.
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