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AAV Serotype Blastu2122 Kit

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产品名称: AAV Serotype Blastu2122 Kit
产品型号: AAV099
产品展商: ABM
产品文档: 无相关文档

简单介绍

AAV Serotype Blastu2122 Kit


AAV Serotype Blastu2122 Kit  的详细介绍
Description
  AAV is a small, non-enveloped virus from the family infecting both dividing and non-dividing cells. The choice of cell types, serotypes, and the optimal multiplicity of infection (MOI) play a key role in influencing transduction efficiency during an experiment. Thus, it is crucial to develop the optimal criteria of AAV transduction for your research. Our AAV Serotype Blast™ Kit will provide you the answer you are looking for. This AAV serotype starter kit includes 9 pre-packaged helper-free Adeno-associated viruses with GFP expressions. You can establish all foundation standards with AAV Serotype Blast™ Kit and enhance your research findings in a timely manner. Saving time and extra amount effort, all you need to do is try our kit.


Figure 1: 293T cells were seeded at 20,000 cells/well in 24-well plate. Next day they were transduced with different serotype of AAV at a MOI of 500. Fluorescent images are taken 5 days after infection.This image shows that serotype 1,2 and 3 is best for this cell line while serotype 4-6 show very low transduction efficiency. Thus, AAV Serotype Blast™ Kit can help decide which serotype should be used for delivery of gene in this specific cell line.
Advantages of abm’s AAV Serotype Blast™ Kit
  • Serotype 1-9 available in the kit for testing
  • The kit is easy to use
  • Simplify your transduction optimization process
  • Helps you to draw accurate conclusions on suitable serotypes for your cell lines
  • This AAV serotype starter Kit has a large range of the cell types which can be tested against
  • Saves you time for your research
References:
  1. Ellis, et al. " A survey of ex vivo/in vitro transduction efficiency of mammalian primary cells and cell lines with Nine natural adeno-associated virus (AAV1-9) and one engineered adeno-associated virus serotype." Virology Journal 10(74): (2013).
  2. Schultz, B.R. et al. " Recombinant Adeno-associated Virus Transduction and Integration." The American Society of Gene Therapy 16(7):1189-1199 (2008). DOI: doi:10.1038/mt.2008.103.
  3. Pajusola, K. et al. " Cell-Type-Specific Characteristics Modulate the Transduction Efficiency of Adeno-Associated Virus Type 2 and Restrain Infection of Endothelial Cells." Journal of Virology 76(22):11530-11540 (2002). DOI: 10.1128/JVI.76.22.11530–11540.2002.
  4. Fisher, K.J. et al. "Transduction with Recombinant Adeno-Associated Virus for Gene Therapy Is Limited by Leading-Strand Synthesis." American Society for Microbiology – Journal of Virology 70(1):1520-1532 (1996).
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