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BrightGreen miRNA qPCR MasterMix-No Dye

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产品名称: BrightGreen miRNA qPCR MasterMix-No Dye
产品型号: MasterMix-mS
产品展商: ABM
产品文档: 无相关文档

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BrightGreen miRNA qPCR MasterMix-No Dye


BrightGreen miRNA qPCR MasterMix-No Dye  的详细介绍
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Description BrightGreen miRNA qPCR MasterMix is designed specifically for quantitative real-time analysis of miRNA samples. The components of BrightGreen miRNA qPCR MasterMix have been developed for superb performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. The chemically modified Hotstart Taq polymerase included in our MasterMix significantly reduces non-specific PCR amplification observed with regular Taq polymerase.
Form MasterMix
Protocol Click To Open
Concentration 2X
Storage Upon arrival, BrightGreen miRNA qPCR MasterMix should be stored at -20ºC and protected from light. After each experiment, the leftover thawed mix can be stored at 4ºC if it is to be used within the next 3 months.
Notes Primer concentration should not be high. 100nM to 300nM each
- primer usually gives best results
- Melting curve is useful in distinguishing nonspecific amplification products and primer dimers from desired product. It's recommended to run melting curve following amplification cycles.
- To do replicates
- To reduce pipetting error: preparing amplicon specific master mix, using repeating pipet, and keeping pipetting volume in manufacture suggested range are very effective to get tight Ct.

This product is ISO-13485 certified.
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