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OneScript® Plus Reverse Transcriptase (PCR)

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产品名称: OneScript® Plus Reverse Transcriptase (PCR)
产品型号: G177
产品展商: ABM
产品文档: 无相关文档

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OneScript® Plus Reverse Transcriptase (PCR)


OneScript® Plus Reverse Transcriptase (PCR)  的详细介绍
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Description Synthesize cDNA from complex RNA templates (i.e. secondary structures and high GC content) with abm’s OneScript® Plus Reverse Transcriptase. Engineered to perform under high temperatures (45°C - 55°C), abm’s OneScript® Plus Reverse Transcriptase can synthesize full-length cDNA libraries from RNA templates up to 15kb in length. In addition, OneScript® Plus Reverse Transcriptase has outstanding proofreading ability due to the presence of a fidelity-enhancing subunit, thus making this RTase an excellent choice for whole genome sequencing.
Literature Click To Open
Application • Synthesizing cDNA from a ssRNA
• DNA primer extension
• Sequencing dsDNA
• Constructing cDNA library
• Constructing libraries for serial analysis for gene expression (SAGE)
• Synthesizing cDNA in rapid amplification of cDNA ends (3’ & 5’ RACE)
• Producing template for use in RT-PCR or real-time RT-PCR
• Labelling 3'-end of duplex DNA via end-filling reactions
• Generating probes for hybridization
Form Enzyme supplied with 5X RT buffer
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Protocol Click To Open
Concentration 200 U/ul
Storage Store all components at -20°C.
Notes This product is ISO-13485 certified.
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