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ABMgood
One-Step RT-PCR Kit
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产品名称:
One-Step RT-PCR Kit
产品型号:
G174
产品展商:
ABM
产品文档:
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简单介绍
One-Step RT-PCR Kit
One-Step RT-PCR Kit
的详细介绍
Print Version
Description
One reaction tube for RT and PCR now offers unparalleled sensitivity and efficiency.
With
abm
’s One-Step RT-PCR Kit, you can streamline the procedure for reverse transcription and subsequent PCR in the same reaction tube, eliminating the need to change buffers and reducing the risk of contamination. This kit offers the end-user an efficient and easy alternative to the conventional “two-step” RT-PCR approach with extreme sensitivity, specificity and high product yield.
G174-dye buffer contains Cresol Red as an electrophoresis dye, with migration equivalent to 125 bp DNA fragment on an agarose gel.
Literature
Application
• Gene-expression analysis
• Transcription analysis
• Gene cloning
• Multiplex RT-PCR
• High throughput applications
• Virus detection and quantification
Picture 1
Protocol
Storage
Store all components at -20°C.
Notes
• Multiple freezing and thawing of RNA should be avoided. Thaw and keep RNA on ice.
• It is recommended that the One-Step RT-PCR is setup under conditions where RNase contamination has been eliminated. Pipette tips and tubes should be treated with 0.1% DEPC.
• Wearing gloves when performing procedure is highly recommended.
This product is ISO-13485 certified.
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