5X All-In-One RT MasterMix (with ExCellenCT Lysis Kit)

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产品名称: 5X All-In-One RT MasterMix (with ExCellenCT Lysis Kit)

产品型号: G916

产品展商: ABM

产品文档: 无相关文档

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5X All-In-One RT MasterMix (with ExCellenCT Lysis Kit)

5X All-In-One RT MasterMix (with ExCellenCT Lysis Kit) 的详细介绍

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Description	Extracting and preparing RNA templates directly from cultured cells to be reverse-transcribed into cDNA, with 5X All-In-One RT MasterMix. abm’s ExCellenCT Lysis Kit is an unique and effective alternative method to provide RNA templates that are extracted and processed directly from cultured cells in less than 15 minutes, unveiling reverse-transcription-ready RNA/cell lysate from cultured cells without the time-consuming and hazardous-chemicals-involved RNA extraction and purification steps. RNA templates generated can then be directly used as template to be reverse-transcribed into cDNA using the 5X All-In-One RT MasterMix. The 5X All-In-One RT MasterMix is a ready-to-use formulation of all the reagents necessary for first-strand cDNA synthesis, including abm’s proprietary OneScript^® Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, dNTPs, and a finely balanced ratio of Oligo (dT)s and Random Primers. Coupled together, this complete system provides the ultimate convenience in generating high-quality cDNA directly from 10-105 cultured cells, suitable for a wide range of downstream applications. This product is only intended for quick RNA extraction from cultured cells for two-steps RT-PCR or two-steps qRT-PCR gene expression analysis. This product is not suitable for typical RNA/cDNA pool preparation.
Literature
Application	•Gene expression studies
Picture 1
Protocol
Storage	Store all components at -20C
Notes	This product is only intended for quick RNA extraction from cultured cells for two-steps RT-PCR or two-steps qRT-PCR gene expression analysis. This product is not suitable for typical RNA/cDNA pool preparation.

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