5X All-In-One RT MasterMix (with AccuRT Genomic DNA Removal Kit)

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产品名称: 5X All-In-One RT MasterMix (with AccuRT Genomic DNA Removal Kit)

产品型号: G492

产品展商: ABM

产品文档: 无相关文档

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5X All-In-One RT MasterMix (with AccuRT Genomic DNA Removal Kit) 的详细介绍

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Description	Removal of contaminating genomic DNA from your samples to reverse transcription of RNA in 30 minutes, with 5X All-In-One RT MasterMix (AccuRT Genomic DNA Removal Kit included). abm’s 5X All-in-One RT MasterMix (with AccuRT Genomic DNA Removal Kit) offers a convenient and complete system to facilitate the complete elimination of contaminating genomic DNA in RNA preparations for rapid synthesis of high-quality cDNA for downstream applications. Key Features • Efficient ds and ssDNA digestion in under 10 minutes • Maximal RNA protection – No heating or organic extraction steps required • Fewer handling errors, with only 1 liquid transfer step for reverse transcription • Streamlined protocol suitable for high-throughput applications • Simple set-up for any RNA template • High reproducibility and excellent cDNA yield Note - In case of no RT control, it is suggested to take a small aliquot of the 5X All-In-One RT MasterMix, and perform deactivation of the RT by incubating the small aliquot on heat block at 85°C for 5 -10 minutes prior to the reaction setup.
Literature
Application	• Generation of templates for use in RT-PCR and qRT-PCR • cDNA synthesis from ssRNA • cDNA library construction • Generation of probes for hybridization • DNA primer extension
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Protocol
Storage	Store all components at -20C
Notes	This product is ISO-13485 certified.

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