SpeedySeq DNA Sequencing with Custom Primer Synthesis

Description

  ABM is proud to launch a new and express DNA sequencing service for our valued customers. This service has been designed for rapid delivery and great value, making your sequence data available for download within a week of your samples being received.
  We employ streamlined technology platforms along with our proprietary reagents for an optimized and highly accurate performance, with on-hand tech support to assist with your primer design and special requirements - You can count on our dedicated and highly experienced team for a reliable service to meet all your sequencing needs.
  This service includes the custom synthesis of one sequencing primer of 20-30bp in length to be used for sequencing your DNA sample. Please include your desired primer sequence at the time of order.

Features/Specifications

Sequencing of templates up to 800bp - results delivered within a week.

Submit a wide range of DNA samples:

purified PCR products

plasmids

genomic DNA

single-stranded Phage DNA

large constructs (DNA, BAC, Cosmids and YAC clones)

We can solve many sequencing related problems, such as multiple hairpin loops and uneven base distributions.

We perform rigorous quality control checks to maintain a reliable service.

How to Use the Service

1.   Download and fill out an order request form. Please include all the required information such as your template name, concentration, estimated size and primer name, plus any special notes (high/low GC, multiple hairpin etc). Please provide your billing information & Email address. You will be sent a unique password to your email to access your sequencing data online.
     Order request form can be attached and emailed to order@abmgood. A confirmation email for shipping your sample will then be sent to your email address. If a primer name is listed on the ABM Sequencing Primer list, these will be provided as part of the service at no additional cost. When choosing our free sequencing primer(s), please use the primer name exactly as shown in the list, and leave the primer concentration cell blank. If they are not listed, they will need to be provided along with your samples.


2. Send your samples (+ Primers where applicable) to us at:
   ABM Inc.
   1-3671 Viking Way
   Richmond, BC
   V6V 2J5 CANADA
   
   

   Template Type	DNA Concentration	DNA Volume	Primer Concentration	Primer Volume
   Plasmid	100 ng/μl	10 μl	5 μM	10 μl
   PCR (100-500bp)	5-10 ng/μl	10 μl	5 μM	10 μl
   PCR (500bp-1Kb)	10-20 ng/μl	10 μl	5 μM	10 μl
   PCR (>1.0Kb)	20-40 ng/μl	10 μl	5 μM	10 μl
   Cosmid	0.5-1 μg/μl	20 μl	5 μM	10 μl
   Fosmid	0.5-1 μg/μl	20 μl	5 μM	10 μl
   BAC	0.5-1 μg/μl	20 μl	5 μM	10 μl
   Genomic DNA	2-3 μg/μl	20 μl	5 μM	10 μl



3. You will receive an email containing your login name and password along with instructions on how to download your sequencing data via the DNA Sequencing Results Login