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T4 DNA Ligase

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产品名称: T4 DNA Ligase
产品型号: G467
产品展商: ABM
产品文档: 无相关文档

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T4 DNA Ligase


T4 DNA Ligase  的详细介绍
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Description T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the proximal 3’- hydroxyl and 5’- phosphate ends of adjacent, nicked nucleotides of dsDNA, dsRNA or DNA-RNA hybrids. Known to proceed via an adenylated intermediate, this endergonic reaction can be utilized to join both cohesive-ended and blunt-ended termini.
Application • Cloning of restriction fragments
• Attaching double-stranded oligonucleotide linkers or adaptors to DNA
• Site-directed mutagenesis
• Nick repair of duplex DNA, RNA or DNA-RNA hybrids
• Self-circularization of linear DNA
Form Enzyme supplied with 5X Reaction Buffer
Image
Concentration 1 U/μl
Storage Store all components at -20°C.
Notes This product is distributed for laboratory research only. Caution: Not for diagnostic use.
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