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DNA Polymerase I Large (Klenow) Fragment

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产品名称: DNA Polymerase I Large (Klenow) Fragment
产品型号: E013
产品展商: ABM
产品文档: 无相关文档

简单介绍

DNA Polymerase I Large (Klenow) Fragment


DNA Polymerase I Large (Klenow) Fragment  的详细介绍
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Description DNA Polymerase I Large (Klenow) Fragment is the large fragment of E. coli DNA Polymerase I (abm Cat. No. G469). The Klenow Fragment retains the DNA-dependent DNA polymerase activity of the E. coli DNA Polymerase I but lacks the 5’→3’ exonuclease activity. With its inherent 3’→5’ exonuclease activity, Klenow possesses the polymerization fidelity of the holoenzyme without degrading 5'-termini.
Application
  • DNA blunting by filling-in 5'-overhangs with unlabeled or labeled dNTPs
  • cDNA second-strand synthesis
  • Generate single-stranded DNA probes using random primers
  • Site-directed DNA mutagenesis using synthetic oligonucleotides
  • Dideoxy DNA sequencing of single- or double-stranded DNA templates
  • 3’→5’ exonuclease activity can blunt a 3’-overhang
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    Form Enzyme supplied with 10X Reaction Buffer
    Image
    Concentration 5 U/μl
    Storage Store all components at -20°C.
    Storage Buffer 25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol.
    Notes One unit is defined as the amount of DNA Polymerase I Large (Klenow) Fragment that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.

    This product is distributed for laboratory research only.
    Caution: Not for diagnostic use.
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