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E. coli DNA Polymerase I

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产品名称: E. coli DNA Polymerase I
产品型号: G469
产品展商: ABM
产品文档: 无相关文档

简单介绍

E. coli DNA Polymerase I


E. coli DNA Polymerase I  的详细介绍
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Description E.coli DNA Polymerase I is a DNA-dependent DNA polymerase with intrinsic 3’→5’ and 5’→3’ exonuclease activities. Nick translation is facilitated by this enzyme due to the removal of nucleotides ahead of the growing chain via 5´→3´ exonuclease activity.
Application
  • Generation of labeled DNA probes by nick translation
  • Second-strand synthesis of cDNA

    • Note: This product is not suitable for NGS or second strand cDNA synthesis applications.
    Form Enzyme supplied with 10X Reaction Buffer
    Image
    Concentration 5 U/μl
    Storage Store all components at -20°C.
    Storage Buffer 25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol.
    Notes One unit is defined as the amount of E. coli DNA Polymerase I that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.

    This product is distributed for laboratory research only.
    Caution: Not for diagnostic use.
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